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KMID : 1024520030120121293
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Journal of the Environmental Sciences
2003 Volume.12 No. 12 p.1293 ~ p.1301
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Identification and Characteristics of a Purple, Non-Sulfur Bacterium, Rhodobacter sp. EGH-24 from Korea Coast
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Cha Mi-Sun
Kim Gi-Han
Cho Sun-Ja
Lee Na-Eun
Lee Jung-Eun
Lee Jae-Dong
Lee Sang-Joon
Bahk Jae-Lim
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Abstract
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A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28-32, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 ％ agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.
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KEYWORD
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Photosynthetic bacterium, PHB, RAPD-PCR, Rhodobacter
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